Immunogen
A synthesized peptide derived from human SOX2
Antibody's target
Human SOX2
Antibody's full description
Rabbit Anti-Human SOX2 Polyclonal
100 µl
SPC-1287D-ALP
350€
A synthesized peptide derived from human SOX2
Human SOX2
Rabbit Anti-Human SOX2 Polyclonal
Stem Cells, Embryonic Stem Cells, ESC Intracellular Markers, Stem Cells, Germline Stem Cells
Polyclonal Antibodies
SOX 2 Antibody, SRY (sex determining region Y) box 2 Antibody, SRY related HMG box 2 Antibody, SRY-box 2 Antibody, ANOP3 Antibody, ysb Antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~34kDa, endogenous levels of total SOX2
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1287 was sufficient for detection of SOX2 in 10 µg of HT29 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus
See included datasheet or contact our support service.
The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development when it forms complexes with Oct4 and Nanog. Varying levels of Sox2 can affect the determination of cell fate. Sox2 inhibits differentiation into the mesendoderm germ layer and promotes differentiation into neural ectoderm germ layer.
1. "SOX2". NCBI. 2. Johansson H, Simonsson S (2010) Aging. (11): 815–822. 3. Thomson M., Liu S.J., Zou L.N., Smith Z., Meissner A., Ramanathan S. (2011) Cell. 145(6): 875–889.
6/Sep/2016
NP_003097.1
6657
P48431
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
