Immunogen
Synthesized peptide derived from internal of human Smad4.
Antibody's target
Human Smad4
Antibody's full description
Rabbit Anti-Human Smad4 Polyclonal
100 µl
SPC-1286D-ALP
350€
Synthesized peptide derived from internal of human Smad4.
Human Smad4
Rabbit Anti-Human Smad4 Polyclonal
Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic, Cardiovascular System, Heart, Cardiogenesis, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
Polyclonal Antibodies
(Small) Mothers Against Decapentaplegic Antibody, Deleted in Pancreatic Carcinoma 4 Antibody, DPC 4 Antibody, hSMAD4 Antibody, JIP Antibody, MADH 4 Antibody, Mothers against decapentaplegic homolog 4 Antibody, SMAD 4 Antibody, SMAD mothers against DPP homolog 4 Antibody
WB, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~60kDa, endogenous levels of total Smad4 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1286 was sufficient for detection of Smad4 in 10 µg of HeLa, Cos7, HepG2, HUVEC cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus
See included datasheet or contact our support service.
Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Smad4 mediates all activities through interaction with various other Smad family members.
1. Heldin C.H. et al. (1997) Nature. 390: 465-71. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-94. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-91. 4. Whitman M. (1998) Genes Dev. 12: 2445-62. 5. Wu G. et al. (2000) Science. 287: 92-7.
6/Sep/2016
NP_005350.1
4089
Q13485
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human, Monkey HeLa, Cos7, HepG2, HUVEC cell lysates showing detection of ~60kDa Smad4 protein using Rabbit Anti-Smad4 Polyclonal Antibody (SPC-1286). Lane 1: Human HeLa. Lane 2: Monkey Cos7. Lane 3: Human HepG2. Lane 4: Human HUVEC. Lane 5: Treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Smad4 Polyclonal Antibody (SPC-1286) at 1:1000. Predicted/Observed Size: ~60kDa. Rabbit Anti-Smad4 Antibody used in Western blot (WB) on HeLa, Cos7, HepG2, HUVEC cell lysates (SPC-1286)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
