Immunogen
A synthesized peptide derived from human Smad2
Antibody's target
Human Smad2
Antibody's full description
Rabbit Anti-Human Smad2 Polyclonal
100 µl
SPC-1284D-ALP
350€
A synthesized peptide derived from human Smad2
Human Smad2
Rabbit Anti-Human Smad2 Polyclonal
Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic
Polyclonal Antibodies
hMAD-2 Antibody, JV18 Antibody, MAD Antibody, MAD homolog 2 Antibody, SMAD 2 Antibody, SMAD family member 2 Antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~58kDa, endogenous levels of total Smad2
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1284 was sufficient for detection of Smad2 in 10 µg of Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus
Expressed at high levels in skeletal muscle, heart and placenta.
Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.
1. Heldin C.H. et al. (1997) Nature. 390: 465-471. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-194. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-791. 4. Whitman M. (1998) Genes Dev. 12: 2445-2462. 5. Wu G. et al. (2000) Science. 287: 92-97. 6. Abdollah S., et al. (1997) J. Biol. Chem. 272: 27678-27685. 7. Souchelnytskyi S., et al. (1997) J. Biol. Chem. 272: 28107-28115. 8. Liu X., et al. (1997) Proc. Natl. Acad. Sci. USA. 94: 10669-10674.
6/Sep/2016
NP_005892.1
4087
Q15796
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human Hek293 cell lysates showing detection of ~52kDa Smad2 protein using Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284). Lane 1: Human Hek293 treated with the immunizing peptide. Lane 2: Human Hek293. Primary Antibody: Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284) at 1:1000. Predicted/Observed Size: ~52kDa. Rabbit Anti-Smad2 Antibody used in Western blot (WB) on Hek293 cell lysates (SPC-1284)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
