Immunogen
A synthesized peptide derived from human CD31
Antibody's target
Human CD31
Antibody's full description
Rabbit Anti-Human CD31 Polyclonal
100 µl
SPC-1283D-ALP
350€
A synthesized peptide derived from human CD31
Human CD31
Rabbit Anti-Human CD31 Polyclonal
Cardiovascular System, Blood, Platelets, Cell Signaling, Cytoskeleton, Cell adhesion, Angiogenesis, Growth Factors, Angiogenic Factors, Stem Cells, Mesenchymal Stem Cells, MSC Surface Molecules, Hematopoietic Progenitors, Lymphoid, T Lymphocytic Lineage, Myeloid, Monocytic Lineage, Stem Cells, Neutrophil Lineage, Thrombocytic Lineage, Cardiovascular Markers, Endothelial Cells, Atherosclerosis, Vascular Inflammation, Leukocyte Recruitment, Cancer, Invasion/microenvironment, Immunology, Cell Type Markers, CD Markers
Polyclonal Antibodies
CD31 Antibody, CD31 antigen Antibody, EndoCAM Antibody, PEC1 Antibody, PECA1 Antibody, PECAM Antibody, PECAM1 Antibody, Platelet endothelial cell adhesion molecule Antibody, GPIIA Antibody
WB, IHC, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total CD31
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1283 was sufficient for detection of CD31 in 10 µg of Jurkat cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Membrane, Cell Junction
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. CD31 is also expressed in certain tumors, including epithelioid hemangioendothelioma, epithelioid sarcoma-like hemangioendothelioma, other vascular tumors, histiocytic malignancies, and plasmacytomas. It is rarely found in some sarcomas, such as Kaposi's sarcoma, and carcinomas. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219. 4. Ganjei-Azar, Parvin (2007). Color Atlas of Immunocytochemistry in Diagnostic Cytology. [New York]: Springer Science+Business Media, LLC. p. 47.
6/Sep/2016
NP_000433.4
5175
P16284
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human Jurkat cell lysates showing detection of ~82kDa CD31 protein using Rabbit Anti-CD31 Polyclonal Antibody (SPC-1283). Lane 1: Human Jurkat Whole Cell lysates treated with the immunizing peptide. Lane 2: Human Jurkat Whole Cell lysates. Primary Antibody: Rabbit Anti-CD31 Polyclonal Antibody (SPC-1283) at 1:1000. Predicted/Observed Size: ~82kDa. Rabbit Anti-CD31 Antibody used in Western blot (WB) on Jurkat cell lysates (SPC-1283)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
