Immunogen
A synthesized peptide derived from human p21 Cip1
Antibody's target
Human p21
Antibody's full description
Rabbit Anti-Human p21 Polyclonal
100 µl
SPC-1281D-ALP
350€
A synthesized peptide derived from human p21 Cip1
Human p21
Rabbit Anti-Human p21 Polyclonal
Cancer, Cell Cycle, Cip/Kip, Cell Signaling, Epigenetics and Nuclear Signalling
Polyclonal Antibodies
CDKN2A Antibody, CDKN2 Antibody, MTS1 Antibody, Cyclin-dependent kinase inhibitor 2A Antibody, isoforms 1/2/3, Cyclin-dependent kinase 4 inhibitor A Antibody, CDK4I Antibody, p16-INK4a Antibody, Multiple tumor suppressor 1 Antibody
WB
Rabbit
Human, Mouse, Rat
WB (1:2000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~21kDa, endogenous levels of total p21 Cip1
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1281 was sufficient for detection of p21 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus
Expressed in all adult human tissues
p21 is a potent cyclin-dependent kinase inhibitor(Cdks). It is a novel Cdk-interacting protein in that it has been identified in cyclin A, cyclin D1, cyclin E and Cdk2 immunoprecipitates. p21 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk 2, cyclin E-Cdk2, cyclin D1-Cdk4 and cyclin D2-Cdk4 complexes. Expression of p21 is inducible by wildtype, but not mutant, p53.
1. "Entrez Gene: CDKN1A cyclin-dependent kinase inhibitor 1A (p21, Cip1)". 2. Harper J.W., Adami G.R., Wei N., Keyomarsi K., Elledge S.J. (1993) Cell. 75(4): 805–816. 3. el-Deiry W.S., et al. (1993). Cell. 75(4): 817–825. 4. Xiong Y., et al. (1993) Nature. 366: 701–704. 5. Rodriguez R., Meuth M. (2006) Mol. Biol. Cell. 17(1): 402–412.
6/Sep/2016
NP_000380.1
1026
P38936
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa cell lysates showing detection of ~26kDa p21 protein using Rabbit Anti-p21 Polyclonal Antibody (SPC-1281). Lane 1: EGF treated Human HeLa whole cell lysates treated with the immunizing peptide. Lane 2: EGF treated Human HeLa whole cell lysates. Primary Antibody: Rabbit Anti-p21 Polyclonal Antibody (SPC-1281) at 1:1000. Predicted/Observed Size: ~26kDa. Rabbit Anti-p21 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1281)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
