Immunogen
A synthesized peptide derived from human NF-kappaB p65
Antibody's target
Human NFkB p65
Antibody's full description
Rabbit Anti-Human NFkB p65 Polyclonal
100 µl
SPC-1279D-ALP
350€
A synthesized peptide derived from human NF-kappaB p65
Human NFkB p65
Rabbit Anti-Human NFkB p65 Polyclonal
Cancer, Apoptosis, Neuroscience, Neurodegeneration, Alzheimer’s Disease, Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, NFkB Pathway, Cardiovascular System, Atherosclerosis, Vascular Inflammation, Inflammatory Mediators, Cell Signaling, Metabolism
Polyclonal Antibodies
NFKB3 Antibody, Nuclear factor NF-kappa-B p65 subunit Antibody, RELA Antibody, TF65 Antibody, Transcription factor p65 Antibody, p65 Antibody
WB, IHC, ICC/IF, IP
Rabbit
Human, Mouse, Rat
WB (1:2000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~69kDa, endogenous levels of total NF-kappaB p65
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1279 was sufficient for detection of NFkB p65 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus, Cytoplasm
Ubiquituous.
NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
1. Baeuerle P.A. and Henkel T. (1994) Annu Rev Immunol. 12: 141-79. 2. Baeuerle P.A. and Baltimore D. (1996) Cell. 87: 13-20. 3. Haskill S., et al. (1991) Cell. 65: 1281-9. 4. Thompson J.E., et al. (1995) Cell. 80: 573-82. 5. Whiteside S.T., et al. (1997) EMBO J. 16: 1413-26.
6/Sep/2016
NP_068810.3
5970
Q04206
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa cell lysates showing detection of ~65kDa NFkB p65 protein using Rabbit Anti-NFkB p65 Polyclonal Antibody (SPC-1279). Lane 1: Human HeLa treated with the immunizing peptide. Lane 2: Human HeLa. Primary Antibody: Rabbit Anti-NFkB p65 Polyclonal Antibody (SPC-1279) at 1:1000. Predicted/Observed Size: ~65kDa. Rabbit Anti-NFkB p65 antibody used in Western blot (WB) on HeLa cell lysates (SPC-1279)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) is a protein complex that controls transcription of DNA, cytokine production and cell survival. NF-κB is found in almost all animal cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. NF-κB plays a key role in regulating the immune response to infection (κ light chains are critical components of immunoglobulins). Incorrect regulation of NF-κB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection, and improper immune development. NF-κB has also been implicated in processes of synaptic plasticity and memory
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
