Immunogen
Synthesized peptide derived from human MMP-2.
Antibody's target
Human MMP 2
Antibody's full description
Rabbit Anti-Human MMP 2 Polyclonal
100 µl
SPC-1278D-ALP
350€
Synthesized peptide derived from human MMP-2.
Human MMP 2
Rabbit Anti-Human MMP 2 Polyclonal
Cardiovascular System, Angiogenesis, Growth Factors, Adhesion / Extracellular Matrix, Matrix Metalloproteinases, Cancer, Invasion/microenvironment, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
Polyclonal Antibodies
72 kDa gelatinase Antibody, CLG 4 Antibody, Collagenase Type 4 alpha Antibody, Matrix metallopeptidase 2 gelatinase A 72kDa Antibody, Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) Antibody, Matrix Metalloproteinase 2 Antibody, MMP II Antibody, MONA Antibody, PEX Antibody, TBE 1 Antibody
WB, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~72kDa, endogenous levels of total MMP-2 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1278 was sufficient for detection of MMP 2 in 10 µg of LOVO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Extracellular matrix, Membrane, Nucleus
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the ability to degrade all the components of the extracellular matrix. Specifically, MMP2, the signaling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity.
1. Cawston T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472. 2. Coussens L.M., et al. (2002) Science. 295: 2387-92. 3. Sternlicht M.D., et al. (1999) Cell. 98: 137-46. 4. Vu T.H., et al. (1998) Cell. 93: 411-22.
6/Sep/2016
NP_004521.1
4313
P08253
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human LOVO cell lysates showing detection of ~72kDa MMP 2 protein using Rabbit Anti-MMP 2 Polyclonal Antibody (SPC-1278). Lane 1: Human LOVO cells. Lane 2: Human LOVO cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-MMP 2 Polyclonal Antibody (SPC-1278) at 1:1000. Predicted/Observed Size: ~72kDa. Rabbit Anti-MMP 2 Antibody used in Western blot (WB) on LOVO cell lysates (SPC-1278)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
