Immunogen
A synthesized peptide derived from human MMP1
Antibody's target
Human MMP 1
Antibody's full description
Rabbit Anti-Human MMP 1 Polyclonal
100 µl
SPC-1277D-ALP
350€
A synthesized peptide derived from human MMP1
Human MMP 1
Rabbit Anti-Human MMP 1 Polyclonal
Cardiovascular System, Angiogenesis, Growth Factors, Adhesion / Extracellular Matrix, Matrix Metalloproteinases, Cell Signaling, Extracellular Matrix (ECM), Enzymes, Cancer, Invasion/microenvironment
Polyclonal Antibodies
27 kDa interstitial collagenase Antibody, CLG Antibody, CLGN Antibody, Matrix metallopeptidase 1 (interstitial collagenase) Antibody, Matrix metalloprotease 1 Antibody, MMP 1 Antibody
WB
Rabbit
Human
WB (1:3000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~54kDa, endogenous levels of total MMP1
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1277 was sufficient for detection of MMP 1 in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Extracellular matrix
Expressed in fibroblasts, keratinocytes, endothelial cells, monocytes, macrophages
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the ability to degrade all the components of the extracellular matrix. MMP1 in particular cleaves collagen types I, II, III and IV, as well as casein, gelatin, antitrypsin, myelin basic protein, L-Selectin, pro-TNF, IL-1 beta, IGF-BP3, IGF-BP5, pro MMP-2 and pro MMP-9. MMP1 is downregulated by p53, and abnormality of p53 expression may lead to joint degradation in rheumatoid arthritis.
1. Cawston T.E. (2004) in Interstitial Collagenase. Barrett, A.J. et al. (eds): Handbook of Proteolytic Enzymes, San Diego: Academic Press, p. 472.
6/Sep/2016
NP_002412.1
4312
P03956
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HepG2 cell lysates showing detection of ~54kDa MMP 1 protein using Rabbit Anti-MMP 1 Polyclonal Antibody (SPC-1277). Lane 1: Human HepG2 cell lysate treated with the immunizing peptide. Lane 2: Human HepG2 cell lysate. Primary Antibody: Rabbit Anti-MMP 1 Polyclonal Antibody (SPC-1277) at 1:1000. Predicted/Observed Size: ~54kDa. Rabbit Anti-MMP 1 Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1277)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
