Immunogen
Synthesized peptide derived from human Collagen IV.
Antibody's target
Human Collagen IV
Antibody's full description
Rabbit Anti-Human Collagen IV Polyclonal
100 µl
SPC-1276D-ALP
350€
Synthesized peptide derived from human Collagen IV.
Human Collagen IV
Rabbit Anti-Human Collagen IV Polyclonal
Stem Cells, Mesenchymal Stem Cells, Chondrogenesis, Cell Signaling, Extracellular Matrix (ECM), Collagen, Cancer, Invasion/microenvironment
Polyclonal Antibodies
Arresten Antibody, CO4A1_HUMAN Antibody, collagen alpha-1(IV) chain Antibody, Collagen Of Basement Membrane Alpha 1 Chain Antibody, Collagen Type IV Antibody
WB, IHC, ELISA
Rabbit
Human, Mouse
WB (1:1000), IHC (1:100), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total Collagen IV protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1276 was sufficient for detection of Collagen IV in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Extracellular matrix
Highly expressed in placenta.
Type-IV collagen is found primarily in the basal lamina. It has been associated with Goodpasture's Disease, as well as Alport Syndrome.
1. Turner N., et al. (1992) J Clin Invest. 89(2): 592–601. 2. "Entrez Gene: COL4A3 collagen, type IV, alpha 3 (Goodpasture antigen)".
6/Sep/2016
NP_001836.3
1282
P02462
Refer to PubMed
To be tested
Immunohistochemistry analysis using Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human HeLa cell lysates showing detection of ~160kDa Collagen IV protein using Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276). Lane 1: Human HeLa cells. Lane 2: Human HeLa cells treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen IV Polyclonal Antibody (SPC-1276) at 1:1000. Predicted/Observed Size: ~160kDa. Rabbit Anti-Collagen IV Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1276) | Rabbit Anti-Collagen IV Antibody used in Western blot (WB) on HeLa cell lysates (SPC-1276)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
