Immunogen
Synthesized peptide derived from internal of human collagen III.
Antibody's target
Human Collagen III
Antibody's full description
Rabbit Anti-Human Collagen III Polyclonal
100 µl
SPC-1275D-ALP
350€
Synthesized peptide derived from internal of human collagen III.
Human Collagen III
Rabbit Anti-Human Collagen III Polyclonal
Cell Signaling, Extracellular Matrix (ECM), Collagen, Stem Cells, Mesenchymal Stem Cells, Osteogenesis, Stem Cells, Mesenchymal Stem Cells, Chondrogenesis
Polyclonal Antibodies
CO3A1_HUMAN Antibody, Collagen alpha 1(III) chain Antibody, Collagen III alpha 1 chain precursor Antibody, Collagen type III alpha 1 Antibody, EDS4A Antibody, Type III collagen Antibody
IHC, ICC/IF, ELISA
Rabbit
Human, Mouse, Rat
IHC (1:100), ICC/IF (1:500), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~138kDa, endogenous levels of total collagen III protein.
-20°C
Blue Ice or 4°C
A 1:100 dilution of SPC-1275 was sufficient for detection of Collagen III in human pancreas tissue using Goat Anti-Rabbit IgG as the secondary antibody.
Extracellular matrix
Soft connective tissues
Type-III collagen is the second most abundant form of collagen in the human body and can be found in intestinal walls, muscles, blood vessels and with collage type-I. Collagen Type-I and Type-III can be mixed together to create supplements for the skin, bone and muscles.
1. Janeczko R.A., Ramirez F. (1989). Nucleic Acids Res. 17(16): 6742. 2. Superti-Furga A., Gugler E., Gitzelmann R., Steinmann B. (1988). J Biol Chem. 263(13): 6226–32. 3. "Entrez Gene: COL3A1 collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant)".
6/Sep/2016
NP_000081.1
1281
P02461
Refer to PubMed
To be tested
Immunohistochemistry analysis using Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275). Tissue: Pancreas Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275). Tissue: HeLa. Species: Human. Primary Antibody: Rabbit Anti-Collagen III Polyclonal Antibody (SPC-1275) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-Collagen III Antibody used in Immunohistochemistry (IHC) on Pancreas Tissue (SPC-1275) | Rabbit Anti-Collagen III Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on HeLa (SPC-1275)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
