Immunogen
Synthesized peptide derived from human Collagen II.
Antibody's target
Human Collagen II
Antibody's full description
Rabbit Anti-Human Collagen II Polyclonal
100 µl
SPC-1274D-ALP
350€
Synthesized peptide derived from human Collagen II.
Human Collagen II
Rabbit Anti-Human Collagen II Polyclonal
Cell Signaling, Extracellular Matrix (ECM), Collagen, Stem Cells, Mesenchymal Stem Cells, Osteogenesis, Cell Signaling, Extracellular Matrix (ECM), Bone
Polyclonal Antibodies
Alpha 1 type II collagen Antibody, AOM Antibody, Cartilage collagen Antibody, Chondrocalcin Antibody, COL11A3 Antibody, Collagen II alpha 1 polypeptide Antibody, SEDC Antibody
WB, IHC, ICC/IF, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total Collagen II protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1274 was sufficient for detection of Collagen II in 10 µg of COLO205 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Extracellular matrix
Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte.
Type-II collagen is the less abundant form of collagen in the human body and can be found in articular and hyaline cartilages and the eyes. It has been associated with the treatment of arthritis, cellulite and wrinkles.
1. Mullazehi M., et al. (2012) Arthritis Research Ther. 14:R100
6/Sep/2016
NP_149162.2
1280
P02458
Refer to PubMed
To be tested
Immunohistochemistry analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Tissue: Cos7. Species: Monkey. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:100. The image on the right is treated with the synthesized peptide. | Western blot analysis of Human COLO205 cell lysates showing detection of ~142kDa Collagen II protein using Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274). Lane 1: Human COLO205. Lane 2: Human COLO205 treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Collagen II Polyclonal Antibody (SPC-1274) at 1:1000. Predicted/Observed Size: ~142kDa. Rabbit Anti-Collagen II Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1274) | Rabbit Anti-Collagen II Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Cos7 (SPC-1274) | Rabbit Anti-Collagen II Antibody used in Western blot (WB) on COLO205 cell lysates (SPC-1274)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
