Immunogen
Synthesized peptide derived from N-terminal of human Collagen I.
Antibody's target
Human Collagen I
Antibody's full description
Rabbit Anti-Human Collagen I Polyclonal
100 µl
SPC-1273D-ALP
350€
Synthesized peptide derived from N-terminal of human Collagen I.
Human Collagen I
Rabbit Anti-Human Collagen I Polyclonal
Cell Signaling, Extracellular Matrix (ECM), Collagen, Stem Cells, Mesenchymal Stem Cells, Osteogenesis
Polyclonal Antibodies
Alpha 1 type I collagen Antibody, Alpha 2 type I collagen Antibody, CO1A1_HUMAN Antibody, collagen alpha 1 chain type I Antibody, EDSC Antibody, Type I procollagen antibody
IHC, ICC/IF, ELISA
Rabbit
Human, Mouse, Rat
IHC (1:100), ICC/IF (1:500), ELISA (1:5000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects ~139kDa, endogenous levels of total Collagen I protein. Collage type I is a triple helix consisting of one alpha-2 and 2 alpha-1 chains, so dimeric (~270kDa) and trimeric (~400kDa) forms may be expected.
-20°C
Blue Ice or 4°C
A 1:100 dilution of SPC-1273 was sufficient for detection of Collagen I in NIH/3T3 cells using Goat Anti-Rabbit IgG as the secondary antibody.
Extracellular matrix
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Type-I collagen is the predominant form of collagen in the human body and plays a role in scar tissue formation. Collagen type 1 is also an important component of tendons and found in most connective tissues including cartilage. Collagen 1 is associated with several diseases including: Ehlers Danlos syndrome (arthrochalasia), Ehlers-Danlos syndrome (classical type), Osteogenesis imperfecta type 1-4 and osteoporosis.
1. Stuart J.M., et al. (2005) Arthritis Rheum. 26(7): 832-840.
6/Sep/2016
NP_000080.2
1278
P08123
Refer to PubMed
To be tested
Immunohistochemistry analysis using Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273). Tissue: Breast Carcinoma Tissue. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273) at 1:100. The image on the right is treated with the synthesized peptide. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273). Tissue: NIH/3T3. Species: Mouse. Primary Antibody: Rabbit Anti-Collagen I Polyclonal Antibody (SPC-1273) at 1:100. The image on the right is treated with the synthesized peptide. Rabbit Anti-Collagen I Antibody used in Immunohistochemistry (IHC) on Breast Carcinoma Tissue (SPC-1273) | Rabbit Anti-Collagen I Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on NIH/3T3 (SPC-1273)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Collagen Types in bones and muscles can be hydrolyzed and used in human grafts. ( Type II )
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
