Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Size

100 µl

Catalog No

SPC-1272D-ALP

Price

350€

Immunogen

A synthesized peptide

Antibody's target

CD9

Antibody's full description

Rabbit Anti- CD9 Polyclonal

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Primary research fields

Immunology, Cell Type Markers, CD Markers, Stem Cells, Embryonic Stem Cells, ESC Surface Molecules

Antibody's category

Polyclonal Antibodies

Antibody's other name

CD9 antigen Antibody, CD9 antigen p24 Antibody, Leukocyte antigen MIC3 Antibody, Motility-related protein Antibody, Tetraspanin-29 Antibody, Tspan-29 Antibody, TSPAN29 Antibody, BA-2/p24 antigen Antibody, BA2 Antibody, Cell growth inhibiting gene 2 protein Antibody, DRAP 27 Antibody, GIG2 Antibody, Growth inhibiting gene 2 protein Antibody

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Verified applications

WB, IHC, ICC/IF

Raised in

Rabbit

Antibody's reactivity

Human, Mouse, Rat

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Antibody's recommended dilutions for use

WB (1:1000), IHC (1:200), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Antibody's concentration

See included datasheet or contact our support service

Antibody's specificity

Detects endogenous levels of total CD9

Storage recommendations

-20°C

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Shipping recommendations

Blue Ice or 4°C

Certificate of analysis

A 1:1000 dilution of SPC-1272 was sufficient for detection of CD9 in 10 µg of COLO cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Membrane

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Tissue specificity

Expressed by a variety of hematopoietic and epithelial cells.

Scientific context

CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins. It plays a role in cell signaling by mediating interactions with low molecular weight GTP-binding proteins.

Bibliography

1. Miao W.M., et al. (2001) Blood. 97:1689. 2. Ellerman D.A., et al. (2003) Mol. Biol Cell. 14: 5098-5103. 3. Schlossman S., et al. (1995) Leucocyte Typing V. Oxford University Press. New York.

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Released date

6/Sep/2016

NCBI number

NP_001760.1

Gene number

928

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Protein number

P21926

PubMed number

Refer to PubMed

Tested applications

To be tested

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Tested reactivity

To be tested

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

No Data Available

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Warning information

Non-hazardous

Country of production

Canada

Total weight (kg)

1.4

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Net weight (g)

0.1

Stock availabilit

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Latin name

Oryctolagus cuniculus

Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase
Rabbit Anti- CD9 Polyclonal: Alkaline Phosphatase