Immunogen
A synthesized peptide derived from human CD34
Antibody's target
Human CD34
Antibody's full description
Rabbit Anti-Human CD34 Polyclonal
100 µl
SPC-1270D-ALP
350€
A synthesized peptide derived from human CD34
Human CD34
Rabbit Anti-Human CD34 Polyclonal
Immunology, Cell Type Markers, CD Markers, Stem Cells, Lineage Markers, Mesoderm, Stem Cells, Mesenchymal Stem Cells, Negative Markers, Stem Cells, Hematopoietic Progenitors, HP Surface Molecules , Stem Cells, Endothelial Progenitors
Polyclonal Antibodies
Hematopoietic progenitor cell antigen CD34 Antibody, CD34 Antibody, Cluster designation 34 Antibody, Mucosialin Antibody, HPCA1 Antibody
WB
Rabbit
Human, Mouse, Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total CD34
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1270 was sufficient for detection of CD34 in 10 µg of HepG2 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Membrane
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
CD34, also known as gp105-120, is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. It is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation sites. It mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
1. Sidney L.E., Branch M.J., Dunphy S.E., Dua H.S., Hopkinson A. (2014). Stem Cells 32(6): 1380–1389. 2. Simmons D.L., Satterthwaite A.B., Tenen D.G., Seed B. (1992). Journal of Immunology. 148 (1): 267–271. 3. Nielsen J.S., McNagny K.M. (2008). Journal of Cell Science 121 (Pt 22): 3683–3692. 4.
6/Sep/2016
NP_001764.1
947
P28906
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HepG2 cell lysates showing detection of ~120kDa CD34 protein using Rabbit Anti-CD34 Polyclonal Antibody (SPC-1270). Lane 1: Human HepG2 cell lysate treated with the immunizing peptide. Lane 2: HumanHepG2 cell lysate. Primary Antibody: Rabbit Anti-CD34 Polyclonal Antibody (SPC-1270) at 1:1000. Predicted/Observed Size: ~120kDa. Rabbit Anti-CD34 Antibody used in Western blot (WB) on HepG2 cell lysates (SPC-1270)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
