Immunogen
Full-length protein.
Antibody's target
Anaplasma marginale mCherry
Antibody's full description
Mouse Anti-mCherry Polyclonal
100 µl
SPC-1268D-ALP
350€
Full-length protein.
Anaplasma marginale mCherry
Mouse Anti-mCherry Polyclonal
Tags and Cell Markers, Fluorescent Proteins
Polyclonal Antibodies
mCherry Antibody, mcherry Antibody
WB, IHC, ICC/IF, IP
Mouse
Species Independent
WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
The mCherry tag antibody can recognize mCherry and mCherry tag fusion proteins.
-20°C
Blue Ice or 4°C
A 1:10000 dilution of SPC-1268 was sufficient for detection of mCherry in 10 µg of mCherry Recombinant Protein by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Contact our support service.
See included datasheet or contact our support service.
mCherry is a fluorescent tracer in transfection and transgenic experiments. Originally it was derived from proteins isolated from Cnidarians (jelly fish, sea anemones and corals), specicially DsRed, a red fluorescent protein from the disc corals of Discosoma species. DsRed is similar in properties to GFP, but, produces a red rather than a green fluoresence. mCherry was engineered with further multiple cycles of mutation, directed modification and evolutionary selection, and in its monomeric form has an excitation maximum at 587 nm and an emission maximum at 610 nm.
1. Shaner N.C., et al. (2004). Nature Biotechnology. 22(12): 1567–1572.
6/Sep/2016
Refer to NCBI
Refer to GenBank
X5DSL3
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of mCherry Recombinant Protein showing detection of ~28kDa mCherry protein using Mouse Anti-mCherry Polyclonal Antibody (SPC-1268). Lane 1: 1:5,000 Dilution. Lane 2: 1:10,000 Dilution. Primary Antibody: Mouse Anti-mCherry Polyclonal Antibody (SPC-1268). Predicted/Observed Size: ~28kDa. Mouse Anti-mCherry Antibody used in Western blot (WB) on mCherry Recombinant Protein (SPC-1268)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.