Immunogen
Synthesized peptide derived from internal of human HMGB1.
Antibody's target
Human HMGB1
Antibody's full description
Rabbit Anti-Human HMGB1 Polyclonal
100 µl
SPC-1259D-ALP
350€
Synthesized peptide derived from internal of human HMGB1.
Human HMGB1
Rabbit Anti-Human HMGB1 Polyclonal
Cell Signaling, Epigenetics and Nuclear Signalling, Transcription, Domain Families, Hmg box
Polyclonal Antibodies
Amphoterin Antibody, Chromosomal protein nonhistone HMG1 Antibody, High mobility group 1 Antibody, High mobility group protein B1 Antibody, HMG-1 Antibody, HMG3 Antibody, HMGB1 Antibody, HMGB1_HUMAN Antibody, SBP 1 Antibody, Sulfoglucuronyl carbohydrate binding protein Antibody
WB, ELISA
Rabbit
Human, Mouse, Rat
WB (1:1000), ELISA (1:20000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of total HMGB1 protein.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1259 was sufficient for detection of HMGB1 in 10 µg of Jurkat, HepG2, COLO, Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus, Chromosome, Cytoplasm, Endosome
Ubiquituous. Expressed in platelets
HMGB1, also known as high mobility group box 1 protein, and functions much like histones as they are important chromatin proteins. In the nucleus, HMGB1 interacts with nucleosomes, transcription factors and histones. The presence of HMGB1 in the nucleus depends on postranslational modifications. Hyperacetylation on lysine residues causes it to translocate into the cytosol. HMGB1 is also secreted by immune cells and acts as a cytokine mediator of inflammation. The mechanism of inflammation and damae is its binding to TLR2. HMGB1 also interacts with P53 and has been proposed as a target for cancer therapy.
1. Bianchi M.E., Agresti A. (2005). Curr. Opin. Genet. Dev. 15(5): 496–506. 2. Klune J.R., Dhupar R., Cardinal J., Billiar T.R., Tsung A. (2008). Mol. Med. 14(7-8): 476–84. 3. Imamura T., Izumi H., Nagatani G., Ise T., Nomoto M., Iwamoto Y., Kohno K. (2001). J. Biol. Chem. 276(10): 7534–40. 4. Lotze M.T., DeMarco R.A. (2003). Curr Opin Investig Drugs. 4(12): 1405–9.
6/Sep/2016
NP_002119.1
3146
P09429
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human Jurkat, HepG2, COLO, Hek293 cell lysates showing detection of ~25kDa HMGB1 protein using Rabbit Anti-HMGB1 Polyclonal Antibody (SPC-1259). Lane 1: Human Jurkat. Lane 2: Human HepG2. Lane 3: Human COLO. Lane 4: Hek293. Lane 5: Treated with the immunizing peptide. Primary Antibody: Rabbit Anti-HMGB1 Polyclonal Antibody (SPC-1259) at 1:1000. Predicted/Observed Size: ~25kDa. Rabbit Anti-HMGB1 Antibody used in Western blot (WB) on Jurkat, HepG2, COLO, Hek293 cell lysates (SPC-1259)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
