Immunogen
A synthesized peptide derived from human Acetyl-Histone H3 around the phosphorylation site of Lys9
Antibody's target
Human Histone H3 (Acetyl pLys9)
Antibody's full description
Rabbit Anti-Human Histone H3 (Acetyl pLys9) Polyclonal
100 µl
SPC-1254D-ALP
350€
A synthesized peptide derived from human Acetyl-Histone H3 around the phosphorylation site of Lys9
Human Histone H3 (Acetyl pLys9)
Rabbit Anti-Human Histone H3 (Acetyl pLys9) Polyclonal
Cell Signaling, Epigenetics and Nuclear Signalling, Histones
Polyclonal Antibodies
H3 histone family, member A Antibody, H3/A Antibody, H31_HUMAN Antibody, H3FA Antibody, Hist1h3a Antibody, histone 1 H3a Antibody, Histone cluster 1 H3a Antibody, Histone H3.1 Antibody
WB
Rabbit
Human, Mouse, Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, pH 7.4, 50% glycerol, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of Acetyl-Histone H3 only when phosphorylated at Lys9.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1254 was sufficient for detection of Histone H3 in 10 µg of HeLa cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus, Chromosome
See included datasheet or contact our support service.
Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1. Histones play a cental role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
1. Wu R., et al. (1981) Cell. 27:321. 2. Strahl B., et al. (2000) Nature. 403:41. 3. Bauer U., et al. (2002) EMBO Rep. 3:39. 4. Frank D., et al. (2003) Gene. 312:135.
6/Sep/2016
NP_003520.1
8350
P68431/Q71DI3/P84243
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa cell lysates showing detection of ~15-17kDa Histone H3 protein using Rabbit Anti-Histone H3 Polyclonal Antibody (SPC-1254). Lane 1: Human HeLa untreated (-). Lane 2: Human HeLa treated with TSA (1µM, 18 hr; +). Primary Antibody: Rabbit Anti-Histone H3 Polyclonal Antibody (SPC-1254) at 1:1000. Predicted/Observed Size: ~15-17kDa. Rabbit Anti-Histone H3 Antibody (Acetyl Lys9) used in Western blot (WB) on HeLa cell lysates (SPC-1254)
Non-hazardous
Canada
1.4
0.1
In Stock
N terminal acetylation or CH3CO as epigenetic regulation of Rabbit Anti- Histone H3 ( pLys9) Polyclonal: Alkaline Phosphatase by NATs.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
