100 µl
SPC-1251D-ALP
350€
Synthesized phosphopeptide derived from human Histone H2A.X around the phosphorylation site of serine 139 (Q-A-SP-Q-E).
Human Histone H2A.X (pSer139)
Rabbit Anti-Human Histone H2A.X (pSer139) Polyclonal
Cell Signaling, Epigenetics and Nuclear Signalling, Histones, DNA/RNA, DNA Damage and Repair, DNA Damage Recognition
Polyclonal Antibodies
H2A histone family member X Antibody, H2A.FX Antibody, H2A.X Antibody, H2AFX Antibody, H2AX histone Antibody, Histone 2A Antibody, Histone 2AX Antibody
WB, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Detects endogenous levels of Histone H2A.X only when phosphorylated at serine 139.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-1251 was sufficient for detection of Histone H2A.X in 10 µg of 293T cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Nucleus, Chromosome
See included datasheet or contact our support service.
H2AX (H2A histone family, member X) is one of several genes coding for histone H2A. H2AX becomes phosphorylated on serine 139 as a reaction on DNA Double-strand breaks (DSB). The kinases of the PI3-family (Ataxia telangiectasia mutated, ATR and DNA-PKcs) are responsible for this phosphorylation, especially ATM. The role of the phosphorylated form potentially allows for space for the recruitment of proteins necessary during repair of DSBs. In addition to its role in DNA-damage repair, H2A.X is required for DNA fragmentation during apoptosis and is phosphorylated by various kinases in response to apoptotic signals. H2A.X is phosphorylated at Ser139 by DNA-PK in response to cell death receptor activation, c-Jun N-terminal Kinase (JNK1) in response to UV-A irradiation, and p38 MAPK in response to serum starvation.
1. Kuo L.J., and Yang L.X. (2008) In Vivo. 22(3): 305-309. 2. Mukherjee B., et al. (2006) DNA Repair (Amst). 5: 575-90. 3. Solier S., et al. (2009) Mol Cell Biol. 29: 68-82. 4. Lu C., et al. (2006) Mol Cell. 23: 121-32. 5. Lu C., et al. (2008) FEBS Lett. 582: 2703-8.
6/Sep/2016
NP_002096.1
3014
P16104
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human 293T cell lysates showing detection of Histone H2A.X protein using Rabbit Anti-Histone H2A.X Polyclonal Antibody (SPC-1251). Lane 1 and Lane 2: Human 293T, Histone2A.X Antibody (Ser139). Lane 3 and Lane 4: Human 293T, Histone2A.X Antibody (pSer139). Primary Antibody: Rabbit Anti-Histone H2A.X Polyclonal Antibody (SPC-1251) at 1:1000. Rabbit Anti-Histone H2A.X Antibody (pSer139) used in Western blot (WB) on 293T cell lysates (SPC-1251)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
