Immunogen
Full-length GFP protein.
Antibody's target
GFP
Antibody's full description
Mouse Anti-GFP Polyclonal
100 µl
SPC-1250D-ALP
350€
Full-length GFP protein.
GFP
Mouse Anti-GFP Polyclonal
Tags and Cell Markers, Fluorescent Proteins
Polyclonal Antibodies
GFP Antibody, Green fluorescent protein Antibody, yfp Antibody
WB, IHC, ICC/IF, IP
Mouse
Species Independent
WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
See included datasheet or contact our support service
Recognizes GFP, EGFP and GFP, EGFP tag fusion proteins. It may also recognize RFP, YFP, CFP.
-20°C
Blue Ice or 4°C
A 1:10000 dilution of SPC-1250 was sufficient for detection of GFP in 10 µg of GFP Protein by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
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Photocytes.
Green Fluorescent Protein (GFP) is a 27 kDa protein produced from the jellyfish Aequorea victoria, and is excited by and brightly fluoresces green (emission peak at a wavelength of 509nm) when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.
1. Prendergast F.G. and Mann K.G. (1978) Biochemistry. 17 (17): 3448–53. 2. Tsien R.Y. (1998) Annual Review of Biochemistry. 67: 509–44.
6/Sep/2016
Refer to NCBI
Refer to GenBank
Refer to Swiss-Prot
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Aequorea victoria GFP Protein showing detection of ~26.9kDa GFP protein using Mouse Anti-GFP Polyclonal Antibody (SPC-1250). Lane 1: 1:5,000 Dilution. Lane 2: 1:10,000 Dilution. Primary Antibody: Mouse Anti-GFP Polyclonal Antibody (SPC-1250). Predicted/Observed Size: ~26.9kDa. Mouse Anti-GFP Antibody used in Western blot (WB) on GFP Protein (SPC-1250)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Mus musculus
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.