Immunogen
Synthetic peptide of human PDGFR alpha (AA322-336)
Antibody's target
Human PDGFR alpha
Antibody's full description
Rabbit Anti-Human PDGFR alpha Polyclonal
100 µl
SPC-1184D-ALP
391€
Synthetic peptide of human PDGFR alpha (AA322-336)
Human PDGFR alpha
Rabbit Anti-Human PDGFR alpha Polyclonal
Polyclonal Antibodies
PDGF alpha chain Antibody, PDGF Receptor alpha Antibody, PDGF-R-alpha Antibody, PDGFR 2 Antibody, PDGFR alpha Antibody, PDGFR-alpha Antibody, PDGFR2 Antibody, PDGFRA Antibody, PDGFR-A Antibody, PDGFRA/BCR fusion Antibody, PGFRA_HUMAN Antibody, Platelet derived growth factor receptor 2 Antibody, Platelet derived growth factor receptor alpha Antibody, Platelet derived growth factor receptor alpha polypeptide Antibody, Platelet derived growth factor receptor Antibody, Alpha-type platelet-derived growth factor receptor Antibody, CD140 antigen-like family member A Antibody, CD140a Antibody, CD140a antigen Antibody, MGC74795 Antibody, Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein Antibody, RHEPDGFRA Antibody
WB, AM
Rabbit
Human, Mouse
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 122.67 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-1184 was sufficient for detection of PDGFR alpha in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cell Membrane, Nucleus
Widely expressed. Detected in platelets, brain, fibroblasts, smooth muscle, heart, and embryo. Expressed in primary and metastatic colon tumors and in normal colon tissue.
PDGFRA is a receptor-tyrosine kinase that is activated upon binding platelet-derived growth factor (PDGF). Ligand binding causes dimerization of the receptor to activate signalling cascades to activate effector substrates such as PIK3R1, PLCG1, and PTPN11. Effector substrates will cause release of calcium from endoplasmic reticulum, by PLCG1, and activation of AKT1, by PIK3R1. This ultimately leads to growth and proliferation in cells by the MAPK and STAT transcription factor activation. PDGFRA is negatively regulated by protein phosphatases to cause inactivation and internalization. Due to PDGFRA's role in cellular growth its dysfunction may lead tumourigenesis. A high number of gastrointestinal stromal tumours have been implicated in PDGFRA dysfunction and constitutive activation. Typically mis-sense or deletion mutations were found to cause constitutively activated PDGFRA. Furthermore, a fusion protein of PDGFRA with BCR can arise from a translocation event with the PDGFRA gene locus. This fusion protein has been implicated in the development of chronic myeloid leukemias.
Refer to PubMed
29/Aug/2016
NP_006197
5156
P16234
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
The Rabbit Anti- PDGFR alpha Polyclonal: Alkaline Phosphatase is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus