Immunogen
Synthetic peptide of human ERK2 (AA320-334)
Antibody's target
Human ERK2
Antibody's full description
Rabbit Anti-Human ERK2 Polyclonal
100 µl
SPC-1144D-ALP
391€
Synthetic peptide of human ERK2 (AA320-334)
Human ERK2
Rabbit Anti-Human ERK2 Polyclonal
Polyclonal Antibodies
ERK Antibody, ERT1 Antibody, ERK2 Antibody, Extracellular Signal Regulated Kinase 2 Antibody, MAP kinase 1 Antibody, MAP kinase 2 Antibody, MAP kinase isoform p42 Antibody, MAPK1 Antibody, MAPK2 Antibody, Mitogen-activated protein kinase 1 Antibody, Mitogen-activated protein kinase 2 Antibody, MK01_HUMAN Antibody, P38 Antibody, P40 Antibody, P41 Antibody, P42MAPK Antibody, PRKM1 Antibody, PRKM2 Antibody protein kinase, protein tyrosine kinase ERK2 Antibody
WB, AM
Rabbit
Human, Mouse
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 42 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-1144 was sufficient for detection of ERK2 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cytoskeleton, Spindle, Nucleus
See included datasheet or contact our support service.
ERK2 or MAPK1 is a protein-serine/threonine kinase. Phosphorylates many diffrent transcription factors, such as ELK1. Acts as transcriptional repressor by binding directly to DNA. Essential for cyclin D1 induction. MAPK1 phosphoryltaes BCL2, which contributes to cell survival, the suppression of the apoptotic effect of BAD and up-regulation of the antiapoptotic protein MCL-1. Regulates accumulation of p53 during DNA damage response. Constitutively active in many human tumours, supposedly due to altered RAS, RAF, EGFR or other upstream elements. ERK/MAPK pathway was shown to promote cell motilty and tumour cell migration.
Refer to PubMed
29/Aug/2016
NP_620407
5594
P28482
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus