Immunogen
Synthetic peptide of human BRAF (AA447-461)
Antibody's target
Human BRAF
Antibody's full description
Rabbit Anti-Human BRAF Polyclonal
100 µl
SPC-1110D-ALP
391€
Synthetic peptide of human BRAF (AA447-461)
Human BRAF
Rabbit Anti-Human BRAF Polyclonal
Polyclonal Antibodies
94 kDa B raf protein antibody, B raf 1 antibody, B Raf proto oncogene serine threonine protein kinase antibody, B RAF1 antibody, B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody, BRAF 1 antibody, BRAF antibody, BRAF_HUMAN antibody, BRAF1 antibody, Murine sarcoma viral (v-raf) oncogene homolog B1 antibody, NS7 antibody, p94 antibody, Proto-oncogene B-Raf antibody, Proto-oncogene c-Rmil antibody, RAFB 1 antibody, RAFB1 antibody, RMIL antibody, Serine/threonine-protein kinase B-raf antibody, v raf murine sarcoma viral oncogene homolog B antibody
WB, AM
Rabbit
Human, Mouse
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 84.491 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-1110 was sufficient for detection of BRAF in 10 µg of human liver lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Nucleus, Cytoplasm, Cell membrane
Brain and testis
BRAF is a serine/threonine kinase that participates in the RAS>RAF>MEK>ERK/MAPK pathway, which is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Ras isoforms are inactive when bound to GDP, but when they are bound to GTP they become active and promote phosphorylation and activation of B-Raf. BRAF is expressed in most tissues with high expression in neuronal tissue. Several genes have been found to be activated by this pathway, among them, cyclins D1, D2 and D3, VEGF, c-myc, b3-integrin and mdm2. BRAF is frequently mutated in human cancers (e.g. sarcomas, colorectal adenocarcinomas, metastatic melanomas, ovarian serous carcinomas, pilocytic astrocytomas); out of 153,189 human tumours, 20.2% featured RafB mutations primarily with the V600E substitutions. This mutation results in constitutive and elevated kinase catalytic activity.
Refer to PubMed
29/Aug/2016
NP_004324
673
P15056
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus