Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Size

100 µl

Catalog No

SPC-1100D-ALP

Price

391€

Immunogen

Synthetic peptide of human Akt1 (AA133-147)

Antibody's target

Human AKT1

Antibody's full description

Rabbit Anti-Human AKT1 Polyclonal

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Antibody's category

Polyclonal Antibodies

Antibody's other name

AKT1 Antibody, AKT1_Human Antibody, PKB Antibody, Protein Kinase B Alpha Antibody, RAC Antibody

Verified applications

WB, AM

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Raised in

Rabbit

Antibody's reactivity

Human, Mouse

Antibody's recommended dilutions for use

WB (1:250); optimal dilutions for assays should be determined by the user.

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS pH7.4, 50% glycerol, 0.025% Thimerosal

Antibody's concentration

See included datasheet or contact our support service

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Antibody's specificity

Detects 55.716 kDa.

Storage recommendations

-20°C

Shipping recommendations

Blue Ice or 4°C

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Certificate of analysis

A 1:250 dilution of SPC-1100 was sufficient for detection of AKT1 in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Cytoplasm, Nucleus, Cell Membrane

Tissue specificity

Expressed in prostate cancer and levels increase from the normal to the malignant state (at protein level). Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages.

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Scientific context

AKT1 is a protein-serine/threonine kinase which is inactive in the cytoplasm. When PI3K is activated by extracellular growth factors, AKT1 is recruited to the cell membrane and phosphorylated to become active. This leads to the induction of survival, proliferation, angiogenesis and metabolism pathways. AKT1 is also involved in cell-cycle progression via its inhibitory phosphorylation of cyclin-dependent kinases and activatory phosphorylation of mTOR. Upregulation of the PI3K/AKT pathways has been implicated in a variety of cancers.

Bibliography

Refer to PubMed

Released date

29/Aug/2016

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

NCBI number

NP_005154

Gene number

207

Protein number

P31749

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

PubMed number

Refer to PubMed

Tested applications

To be tested

Tested reactivity

To be tested

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

No Data Available

Warning information

Non-hazardous

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Country of production

Canada

Total weight (kg)

1.4

Net weight (g)

0.1

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Stock availabilit

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase

Latin name

Oryctolagus cuniculus

Rabbit Anti-Human AKT1 Polyclonal: Alkaline Phosphatase