Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser212 of human MSK1 (AA209-215)
Antibody's target
Human MSK1 (pSer212)
Antibody's full description
Rabbit Anti-Human MSK1 (pSer212) Polyclonal
100 µl
SPC-1031D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Ser212 of human MSK1 (AA209-215)
Human MSK1 (pSer212)
Rabbit Anti-Human MSK1 (pSer212) Polyclonal
Polyclonal Antibodies
90 kDa ribosomal protein S6 kinase 5 antibody, Mitogen and stress activated protein kinase 1 antibody, MSPK1 antibody, Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody, Nuclear mitogen- and stress-activated protein kinase 1 antibody, Ribosomal protein S6 kinase 90kD polypeptide 5 antibody, Ribosomal protein S6 kinase 90kDa antibody, Ribosomal Protein S6 Kinase Alpha 5 antibody, RLPK antibody, RPS6KA5 antibody, RSK Like Protein Kinase antibody, RSK-like protein kinase antibody, RSKL antibody, S6K alpha 5 antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 89.865 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-1031 was sufficient for detection of MSK1 (pSer212) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Nucleus, Cytoplasm
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential. Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumour formation. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines. Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors. Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury.
Refer to PubMed
29/Aug/2016
NP_004746
9252
O75582
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus