Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser821 and Tyr823 of human c-Kit (AA818-827)
Antibody's target
Human c-Kit (Ser821+ Tyr823)
Antibody's full description
Rabbit Anti-Human c-Kit (Ser821+ Tyr823) Polyclonal
100 µl
SPC-1003D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Ser821 and Tyr823 of human c-Kit (AA818-827)
Human c-Kit (Ser821+ Tyr823)
Rabbit Anti-Human c-Kit (Ser821+ Tyr823) Polyclonal
Polyclonal Antibodies
C Kit Antibody, CD117 Antibody, Kit Antibody, KIT oncogene Antibody, Mast cell growth factor receptor Antibody, p145 c-kit Antibody, PBT Antibody, Proto oncogene tyrosine protein kinase Kit Antibody, SCF Receptor Antibody, soluble KIT variant 1 Antibody, Steel Factor Receptor Antibody, tyrosine protein kinase Kit Antibody, v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog Antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 109.865 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-1003 was sufficient for detection of c-Kit (Ser821+ Tyr823) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cell Membrane
Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells
c-KIT is a receptor tyrosine kinase that plays an essential role in the regulation of cell survival and proliferation, especially hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and melanogenesis. Following activation by KITLG/SCF binding, it can activate the PLCG1, AKT1, RAS, and STAT pathways. Activation of PLCG1 can lead to increased calcium signalling. Mutations leading to constitutive kinase activity have been associated with gastrointestinal stromal tumour, testicular tumours, and acute myelogenous leukemia. The human proto-oncogene c-kit was first identified as the homolog of the feline sarcoma viral oncogene v-kit.
Refer to PubMed
29/Aug/2016
NP_000213
3815
P10721
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus