Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Immunogen

A phospho-specific peptide corresponding to residues surrounding Tyr647 and Tyr648 of human FGFR3 (AA644-651)

Antibody's target

Human FGFR2 (pTyr656 + pTyr657)

Antibody's full description

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Antibody's category

Polyclonal Antibodies

Antibody's other name

ACH Antibody, CD333 Antibody, FGFR3 Antibody, CEK2 Antibody, Fibroblast growth factor receptor 3 Antibody, Heparin binding growth factor receptor Antibody, HSFGFR3EX Antibody, Hydroxyaryl protein kinase Antibody, JTK4 Antibody, MFR 3 Antibody, SAM 3 Antibody, Tyrosine kinase JTK 4 Antibody, Tyrosine kinase JTK4 Antibody, Z FGFR 3 Antibody

Verified applications

WB, AM

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Raised in

Rabbit

Antibody's reactivity

Human

Antibody's recommended dilutions for use

WB (1:250); optimal dilutions for assays should be determined by the user.

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS pH7.4, 50% glycerol, 0.025% Thimerosal

Antibody's concentration

See included datasheet or contact our support service

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Antibody's specificity

Detects 87.710 (110-120) kDa.

Storage recommendations

-20°C

Shipping recommendations

Blue Ice or 4°C

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Certificate of analysis

A 1:250 dilution of SPC-983 was sufficient for detection of FGFR2 (pTyr656 + pTyr657) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Cell Membrane, Cytoplasmic Vesicle, Endoplasmic Reticulum

Tissue specificity

Expressed in brain, kidney and testis

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Scientific context

FGFR3 is a receptor-tyrosine kinase that is activated upon binding fibroblast growth factor (FGF). Extracellular ligand binding induces homo- or hetero- dimerization of FGFR to activate signalling. FGFRs have an essential role in regulating cellular differentiation, proliferation and apoptosis. The FGFR3 isoform is specifically involved in chondrocyte development which is physiologically linked to normal skeleton development. Defects in FGFR3 can lead to dwarfism. Overexpressed FGFR3 can promote activation of SHP2 and STAT signalling pathways.Mutations which promote constitutive dimerization of FGFR and kinase activation or impairs internalization and degradation of the receptor have been observed in bladder, skin and oral cancers.

Bibliography

Refer to PubMed

Released date

29/Aug/2016

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

NCBI number

NP_000133

Gene number

2261

Protein number

P22607

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

PubMed number

Refer to PubMed

Tested applications

To be tested

Tested reactivity

To be tested

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

No Data Available

Warning information

Non-hazardous

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Country of production

Canada

Total weight (kg)

1.4

Net weight (g)

0.1

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Stock availabilit

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase

Latin name

Oryctolagus cuniculus

Rabbit Anti-Human FGFR2 (pTyr656 + pTyr657) Polyclonal: Alkaline Phosphatase