Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr577 of human FAK (AA574-580)
Antibody's target
Human FAK (pTyr577)
Antibody's full description
Rabbit Anti-Human FAK (pTyr577) Polyclonal
100 µl
SPC-978D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Tyr577 of human FAK (AA574-580)
Human FAK (pTyr577)
Rabbit Anti-Human FAK (pTyr577) Polyclonal
Polyclonal Antibodies
FADK1 Antibody, FADK Antibody, FAK related non kinase polypeptide Antibody, FAK1 Antibody, FAK Antibody, Focal adhesion kinase 1 Antibody, Focal adhesion kinase isoform FAK Del33 Antibody, Focal adhesion kinase related nonkinase Antibody, FRNK Antibody, p125FAK Antibody, PPP1R71 Antibody, Protein phosphatase 1 regulatory subunit 71 Antibody, Protein tyrosine kinase 2 Antibody, Ptk2 Antibody, PTK2 protein tyrosine kinase 2 Antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 119.233 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-978 was sufficient for detection of FAK (pTyr577) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cell Junctions, Focal Adhesion, Cell Membrane
Highly expressed in brain but found in heart, placenta, lung, kidney and skeletal muscle.
FAK, also known as PTK2, is a non-receptor protein-tyrosine kinase that is activated by tyrosine-phosphorylation in response to integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands. It serves as adaptor protein. It is linked to the MAPK pathway via Grb2, and is also linked to PI3K and PLC gamma pathways. It plays a role as a scaffold for MDM2-mediated p53 degradation, and has been implicated in regulation of cell-cell contacts, microtubule stability and control of gene transcription. FAK has high expression levels in various human cancers as it promotes tumour cell motility, matrix degradation, angiogenesis and suppresses apoptosis, anoikis and senescence.
Refer to PubMed
29/Aug/2016
NP_722560
5747
Q05397
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus