100 µl
SPC-977D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Thr219 and Tyr221 of human ERK2 (AA 216-222)
Human ERK5 (pThr219 + pTyr221)
Rabbit Anti-Human ERK5 (pThr219 + pTyr221) Polyclonal
Polyclonal Antibodies
Big MAP kinase 1 Antibody, BMK 1 kinase Antibody, ERK5 Antibody, BMK1 Antibody, EC 2.7.11.24 Antibody, ERK4 Antibody, ERK5 Antibody, Extracellular signal regulated kinase 5 Antibody, MAP kinase 7 Antibody, MAPK7 Antibody , Mitogen activated protein kinase 7 Antibody, MK07_HUMAN Antibody, OTTHUMP00000065906 Antibody, OTTHUMP00000065907 Antibody, PRKM7 Antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 88.637 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-977 was sufficient for detection of ERK5 (pThr219 + pTyr221) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus
Highly expressed in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver
ERK5 or MAPK7 is a protein-serine/threonine kinase. ERK5 is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5). Upregulation of this gene has been found in breast, prostate, and liver cancer.
Refer to PubMed
29/Aug/2016
NP_002740.2
5598
Q13164
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus