Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr892 of human PERK (AA889-895)
Antibody's target
Human PERK (pThr892)
Antibody's full description
Rabbit Anti-Human PERK (pThr892) Polyclonal
100 µl
SPC-963D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Thr892 of human PERK (AA889-895)
Human PERK (pThr892)
Rabbit Anti-Human PERK (pThr892) Polyclonal
Polyclonal Antibodies
Eif2ak3 Antibody, Eukaryotic translation initiation factor 2 alpha kinase 3 Antibody, Heme regulated EIF2 alpha kinase Antibody, HRI Antibody, HsPEK Antibody, Pancreatic eIF2-alpha kinase Antibody, PEK Antibody, PRKR-like Endoplasmic Reticulum kinase Antibody, WRS Antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 125.147 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-963 was sufficient for detection of PERK (pThr892) in 10 µg of HepG2 cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Endoplasmic Reticulum Membrane
See included datasheet or contact our support service.
PERK (eukaryotic translation initiation factor 2-α kinase 3, eIF2α kinase 3, PEK, WRS) is a Ser/Thr protein kinase, type I membrane protein of the endoplasmic reticulum that is abundant in secretory tissues and is inducible by ER-stress. PERK phosphorylates the α subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, a reduction of translation initiation and repression of global protein synthesis. PERK may serve as an effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1. PERK dimerizes with HSPA5/BIP in resting cells and oligomerizes in ER-stressed cells. Perturbation in protein folding in the endoplasmic reticulum (ER) can promote dissociation from HSPA5/BIP and oligomerization, resulting in autophosphorylation and kinase activity induction.
Refer to PubMed
29/Aug/2016
NP_004827
9451
Q9NZJ5
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus