Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser337 and Thr338 of human CLK1 (AA334-341)
Antibody's target
Human CLK1 (pSer337 + pThr338)
Antibody's full description
Rabbit Anti-Human CLK1 (pSer337 + pThr338) Polyclonal
100 µl
SPC-952D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Ser337 and Thr338 of human CLK1 (AA334-341)
Human CLK1 (pSer337 + pThr338)
Rabbit Anti-Human CLK1 (pSer337 + pThr338) Polyclonal
Polyclonal Antibodies
CDC like kinase 1 Antibody, CDC28/CDC2 like kinase Antibody, CLK Antibody, CLK/STY Antibody, CLK1_HUMAN Antibody, Protein tyrosine kinase STY Antibody, STY Antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 57.205 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-952 was sufficient for detection of CLK1 (pSer337 + pThr338) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Nucleus
See included datasheet or contact our support service.
The CDC-like kinase 1 (CLK1) dually phosphorylates serine- and arginine-rich proteins of the spliceosomal complex, which constitutes a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Specifically, CLK1 may mediate the release of specific proteins from nuclear storage sites. Expression of CLK1 may be very low due to a premature stop codon in the mRNA, which leads to nonsense-mediated mRNA decay. CLK1 activity is positively regulated by phosphorylation on either tyrosine residues or serine/thre-onine residues. CLK1 activity is negatively regulated by steric constraints mediated by the N-terminal domain and also by phosphorylation on a subset of serine/threonine residues within the catalytic domain.
Refer to PubMed
29/Aug/2016
NP_001155879
1195
P49759
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus