Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr172 of human CDK4 (AA169-175)
Antibody's target
Human CDK4 (pThr172)
Antibody's full description
Rabbit Anti-Human CDK4 (pThr172) Polyclonal
100 µl
SPC-943D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Thr172 of human CDK4 (AA169-175)
Human CDK4 (pThr172)
Rabbit Anti-Human CDK4 (pThr172) Polyclonal
Polyclonal Antibodies
CDK4_HUMAN Antibody, Cell division kinase 4 Antibody, CMM 3 Antibody, Crk3 Antibody, Cyclin dependent kinase 4 Antibody, Melanoma cutaneous malignant 3 Antibody, p34 cdk4 Antibody, PSK J3 Antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 33.73 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-943 was sufficient for detection of CDK4 (pThr172) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Nucleus, Membrane
See included datasheet or contact our support service.
CDK4 is a protein-serine/threonine kinase, and a part of the protein kinase complex important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. CDK4 is responsible for the phosphorylation of retinoblastoma gene product (Rb) and mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumourigenesis of a variety of cancers, such as cutaneous malignant melanoma type 3 (CMM3).
Refer to PubMed
29/Aug/2016
NP_000066
1019
P11802
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus