Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Immunogen

A phospho-specific peptide corresponding to residues surrounding Ser383 and Ser385 of human CrkRS (AA380-388)

Antibody's target

Human CrkRS (pSer383 + pSer385)

Antibody's full description

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Antibody's category

Polyclonal Antibodies

Antibody's other name

arginine/serine-rich Antibody, Cdc2 related kinase arginine/serine rich Antibody, CDC2 related protein kinase 7 Antibody, Cdk12 Antibody, Cell division cycle 2 related protein kinase 7 Antibody, Cell division protein kinase 12 Antibody, CRK7 Antibody, Pksc Antibody

Verified applications

WB, AM

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Raised in

Rabbit

Antibody's reactivity

Human

Antibody's recommended dilutions for use

WB (1:250); optimal dilutions for assays should be determined by the user.

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS pH7.4, 50% glycerol, 0.025% Thimerosal

Antibody's concentration

See included datasheet or contact our support service

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Antibody's specificity

Detects 164.155 kDa.

Storage recommendations

-20°C

Shipping recommendations

Blue Ice or 4°C

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Certificate of analysis

A 1:250 dilution of SPC-941 was sufficient for detection of CrkRS (pSer383 + pSer385) in 10 µg of 293T cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Nucleus

Tissue specificity

See included datasheet or contact our support service.

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Scientific context

CrkRS (Cdc2-related kinase, arginine/serine-rich, also designated CRK7 and CRKR) is an ubiquitous protein that appears to localize to the nucleus and link transcription and splicing machinery. CrkRS belongs to the serine/threonine protein kinase family and Cdc2/Cdkx subfamily. CrkRS has extensive proline-rich regions that resemble SH3 and WW domain binding sites, and an RS domain that is characteristic of splicing factors. The protein kinase domain of CrkRS is 89% identical to the CHED protein kinase, also designated CDC2L5 and cell division cycle 2-like 5 (cholinesterase-related cell division controller), however outside the kinase domains the two proteins are unique. Cell cycle control kinases can phosphorylate proteins important for differentiation and apoptosis and provide connections between proliferation, differentiation, apoptosis, and neurocytoskeleton dynamics.

Bibliography

Refer to PubMed

Released date

29/Aug/2016

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

NCBI number

NP_057591

Gene number

51755

Protein number

Q9NYV4

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

PubMed number

Refer to PubMed

Tested applications

To be tested

Tested reactivity

To be tested

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

No Data Available

Warning information

Non-hazardous

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Country of production

Canada

Total weight (kg)

1.4

Net weight (g)

0.1

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Stock availabilit

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase

Latin name

Oryctolagus cuniculus

Rabbit Anti-Human CrkRS (pSer383 + pSer385) Polyclonal: Alkaline Phosphatase