Immunogen
A phospho-specific peptide corresponding to residues surrounding Thr286 of human CaMKII (AA282-289)
Antibody's target
Human CaMKII (pThr286)
Antibody's full description
Rabbit Anti-Human CaMKII (pThr286) Polyclonal
100 µl
SPC-931D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Thr286 of human CaMKII (AA282-289)
Human CaMKII (pThr286)
Rabbit Anti-Human CaMKII (pThr286) Polyclonal
Polyclonal Antibodies
Calcium / calmodulin dependent protein kinase type II Antibody, CaM K2 Antibody, CaM kinase 2 alpha Antibody, CaM-KII Antibody, KCC2A_HUMAN Antibody, CAMK2B Antibody, CAMK2D Antibody, CAMK2G Antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 54.03 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-931 was sufficient for detection of CaMKII (pThr286) in 10 µg of rat brain lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cell Junctions, Synapse, Presynaptic Cell Membrane
See included datasheet or contact our support service.
CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+ auto inhibitory effect and activates the kinase (8). /calmodulin to its regulatory domain releases its This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
1. Hughes K. et al. (2001) J. Biol. Chem. 276: 36008–36013. 2. Barria A. et al. (1997) Science 276: 2042–2045. 3. Bennet M.K. and Kennedy M.B. (1987) Proc. Natl. Acad. Sci. U.S.A. 84: 1794-1798. 4. Broke L., Srinivasan M. and Schulman H. (1995) J. Neurosci. 15: 6797-6808. 5. Nghiem P., Saati S. M., Martens C. L., Gardner P. and Schulman H. (1993) J. Biol. Chem. 268: 5471-5479. 6. Edman C.F. and Schulman H. (1994) Biochem. Biophys. Acta 1221: 90-102. 7. Tombes R.M. and Krystal G.W., (1997) Biochem. Biophys. Acta 13555: 281-292. 8. Means A.R. (2000) Mol. Endocrinol. 14: 4–12. 9. Makhinson M. et al. (1999) J. Neurosci. 19: 2500–2510. 10. Strack S. and Colbran R.J. (1998) J. Biol. Chem. 273: 20689-20692. 11. Leonard S.A., Lim I.A., Hemsworth D.E., Horne M.C. and Hell J.W. (1999) Proc. Natl. Acad. Sci. U.S.A. 96: 3239-3244. 12. Shen K. and Meyer Y. (1999) Science 284: 162-167. 13. Shifman J. M., Choi M. H., Mihalas S, Mayo S. L., Kennedy M. B. 2006 Proc. Natl. Acad. Sci. U.S.A. 103: 13968-13973.
29/Aug/2016
NP_741960
815
Q9UQM7
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus