Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr223 and Tyr225 of human BTK (AA220-226)
Antibody's target
Human BTK (pTyr223 + pTyr225)
Antibody's full description
Rabbit Anti-Human BTK (pTyr223 + pTyr225) Polyclonal
100 µl
SPC-928D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Tyr223 and Tyr225 of human BTK (AA220-226)
Human BTK (pTyr223 + pTyr225)
Rabbit Anti-Human BTK (pTyr223 + pTyr225) Polyclonal
Polyclonal Antibodies
Agammaglobulinaemia tyrosine kinase Antibody, AGMX1 Antibody, ATK Antibody, B cell progenitor kinase Antibody, Bruton tyrosine kinase Antibody, IMD 1 Antibody, PSCTK1 Antibody, Tyrosine protein kinase BTK Antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 76.281 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-928 was sufficient for detection of BTK (pTyr223 + pTyr225) in 10 µg of HEK293 cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Membrane, Nucleus
See included datasheet or contact our support service.
BTK is a cytoplasmic protein-tyrosine kinase, involved in B cell development, differentiation and signalling. It is a downstream target of the B-cell receptor and plays an important role in immune cell related pathways. BTK has a dual function in apoptosis. Not reported to directly interact with DNA. Expression of a truncated BTK variant in leukemia, which mediates phosphorylation and activation of normal BTK by BCR-ABL1. Constitutively activated BTK promotes cell survival.
Refer to PubMed
29/Aug/2016
NP_000052
695
Q06187
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus