Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Size

100 µl

Catalog No

SPC-920D-ALP

Price

391€

Immunogen

A phospho-specific peptide corresponding to residues surrounding Ser227 of human Aurora B (AA222-228)

Antibody's target

Human Aurora B (pSer227)

Antibody's full description

Rabbit Anti-Human Aurora B (pSer227) Polyclonal

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Antibody's category

Polyclonal Antibodies

Antibody's other name

AIK2 Antibody, ARK2 Antibody, Aurora related Kinase 2 Antibody, IPL1 Antibody, STK1 Antibody, Aurora 1 Antibody

Verified applications

WB, AM

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Raised in

Rabbit

Antibody's reactivity

Human

Antibody's recommended dilutions for use

WB (1:250); optimal dilutions for assays should be determined by the user.

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Antibody's purified from

Peptide Affinity Purified

Recommended buffer for storage

PBS pH7.4, 50% glycerol, 0.025% Thimerosal

Antibody's concentration

See included datasheet or contact our support service

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Antibody's specificity

Detects 39.28 kDa.

Storage recommendations

-20°C

Shipping recommendations

Blue Ice or 4°C

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Certificate of analysis

A 1:250 dilution of SPC-920 was sufficient for detection of Aurora B (pSer227) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Antibody in cell

Nucleus, Cytoplasm, Cytoskeleton

Tissue specificity

High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Scientific context

Aurora B is part of the larger AURA family of serine/threonine kinases and is a key regulator of mitotic cell division. It is involved in regulating microtubule formation and the assembly of the mitotic spindle responsible for chromosome segragation during mitosis. AURA activity is cell cycle dependent with the greatest activity occuring during mitosis. The overexpression of AURA has been well documented in many malignant cancers including breast, ovarian, colon, prostate and neuroblastomas. Overexpression of AURA appears to override checkpoints in cell cycle progression leading to increased cell division and proliferation.

Bibliography

Refer to PubMed

Released date

29/Aug/2016

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

NCBI number

NP_004208

Gene number

9212

Protein number

Q96GD4

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

PubMed number

Refer to PubMed

Tested applications

To be tested

Tested reactivity

To be tested

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Representative figure link

No Data Available

Warning information

Non-hazardous

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Country of production

Canada

Total weight (kg)

1.4

Net weight (g)

0.1

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Stock availabilit

In Stock

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Group

Polyclonals and antibodies

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase

Latin name

Oryctolagus cuniculus

Rabbit Anti-Human Aurora B (pSer227) Polyclonal: Alkaline Phosphatase