Immunogen
A phospho-specific peptide corresponding to residues surrounding Tyr246 of human alpha Actinin (AA243-249)
Antibody's target
Human alpha Actinin (pTyr246)
Antibody's full description
Rabbit Anti-Human alpha Actinin (pTyr246) Polyclonal
100 µl
SPC-909D-ALP
391€
A phospho-specific peptide corresponding to residues surrounding Tyr246 of human alpha Actinin (AA243-249)
Human alpha Actinin (pTyr246)
Rabbit Anti-Human alpha Actinin (pTyr246) Polyclonal
Polyclonal Antibodies
ACTN1 Antibody, Actinin alpha1 Antibody, F-actin cross linking protein Antibody, Alpha-actinin cytoskeletal isoform Antibody
WB, AM
Rabbit
Human
WB (1:250); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
See included datasheet or contact our support service
Detects 103.058 kDa.
-20°C
Blue Ice or 4°C
A 1:250 dilution of SPC-909 was sufficient for detection of alpha Actinin (pTyr246) in 10 µg of HeLa cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cytoskeleton, Myofibril, Sarcomere
See included datasheet or contact our support service.
Alpha Actinin belongs to the spectrin gene family which encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures.
Refer to PubMed
29/Aug/2016
NP_001093
87
P12814
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
No Data Available
Non-hazardous
Canada
1.4
0.1
In Stock
The Rabbit Anti- alpha Actinin (pTyr246) Polyclonal: Alkaline Phosphatase is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus