Immunogen
Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
Antibody's target
Pseudomonas aeruginosa Metallothionein
Antibody's full description
Rabbit Anti-Pseudomonas aeruginosa Metallothionein Polyclonal
100 µg
SPC-717D-ALP
391€
Synthetic peptide from the mid-protein of Pseudomonas aeruginosa Metallothionein
Pseudomonas aeruginosa Metallothionein
Rabbit Anti-Pseudomonas aeruginosa Metallothionein Polyclonal
Cell Signaling, Epigenetics and Nuclear Signalling, Transcription, Domain Families, Zinc finger
Polyclonal Antibodies
CES 1 antibody, CES1 antibody, Metallothionein 1A antibody, Metallothionein 1S antibody, Metallothionein 2 antibody, Metallothionein 2A antibody, Metallothionein IA antibody, Metallothionein II antibody, Metallothionein-1A antibody, Metallothionein-IA antibody, Metallothionein2 antibody, MGC32848 antibody, MT 1A antibody, MT 2 antibody, MT 2A antibody, MT IA antibody, MT II antibody, MT-1A antibody, MT-IA antibody, MT1 antibody, MT1A antibody, MT1A_HUMAN antibody, MT1S antibody, MT2 antibody, MT2A antibody, MTC antibody, Probable Metallothionein Antibody
WB
Rabbit
Bacteria (Pseudomonas aeruginosa)
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~36 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-717 was sufficient for detection of Metallothionein in 15 µg of pseudomonas aeruginosa purified Metallothionein (pmtA-GST) protein by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Contact our support service.
See included datasheet or contact our support service.
See included datasheet or contact our support service.
Refer to PubMed
25/Oct/2016
Refer to NCBI
Refer to GenBank
Q9I1X5
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Pseudomonas aeruginosa Purified protein showing detection of ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST) Metallothionein protein using Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717). Lane 1: Molecular Weight Ladder (MW). Lane 2: Blank. Lane 3: Pseudomonas aeruginosa purified protein with GST tag.. Load: 25 µg. Block: 1% milk, 2% BSA. Primary Antibody: Rabbit Anti-Metallothionein Polyclonal Antibody (SPC-717) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Mouse Anti-Rabbit IgG: AP at 1:1000 for 60 min at RT. Color Development: Alkaline phosphate activity colorimetric assay. Predicted/Observed Size: ~36 kDa (9.07 kDa Metallothionein and 26.4 kDa GST). Courtesy of: Michael Lynes and Amy Thees, University of Connecticut. Rabbit Anti-Metallothionein Antibody used in Western blot (WB) on Purified protein (SPC-717)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Pseudomonas is a Gram-negative bacteria. Pseudomonas genomic sequences are used in medical research. A problem in hostpital is often the Pseudomonas Aeruginosa containing multi drug resistancy properties that are transmitted through plasmids. The MDR property is transmitter by the R plasmid.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
