Immunogen
Synthetic peptide of Human calcium-sensing receptor (CASR)
Antibody's target
Human Calcium Sensing Receptor
Antibody's full description
Rabbit Anti-Human Calcium Sensing Receptor Polyclonal
100 µg
SPC-713D-ALP
391€
Synthetic peptide of Human calcium-sensing receptor (CASR)
Human Calcium Sensing Receptor
Rabbit Anti-Human Calcium Sensing Receptor Polyclonal
Neuroscience, Neurotransmitter Receptors, Glutamate Receptors, Cell Signaling, Cytoskeleton, Cancer, Cancer Metabolism
Polyclonal Antibodies
Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism Antibody, HHC Antibody, Extracellular calcium sensing receptor Antibody, FIH Antibody, Parathyroid Cell calcium-sensing receptor Antibody, CASR Antibody, CASR_HUMAN Antibody, Ca sensing receptor Antibody, Extracellular calcium-sensing receptor Antibody, PCAR1 Antibody, FHH Antibody, Ca2+ sensing receptor Antibody, EIG8 Antibody, Hypocalciuric hypercalcemia 1 Antibody, PCaR1 Antibody, GPRC2A Antibody, Extracellular calcium sensing receptor [Precursor] Antibody, PCAR 1 Antibody, Parathyroid cell calcium-sensing receptor 1 Antibody, HHC1 Antibody, Parathyroid Cell calcium sensing receptor Antibody, CAR Antibody, MGC138441 Antibody, NSHPT Antibody, CaSR Antibody, Ca2+ sensing receptor 1 Antibody, Parathyroid Ca(2+) sensing receptor 1 Antibody,
WB, ICC/IF
Rabbit
Human
WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~140 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-713 was sufficient for detection of calcium-sensing receptor (CASR) in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cell Membrane, Multi-Pass Membrane Protein
Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_000379.2
846
P41180
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Calcium Sensing Receptor Antibody (D) Composite. | Western blot analysis of Rat Kidney cell lysates showing detection of 130kDa Calcium Sensing Receptor protein using Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Kidney cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Calcium Sensing Receptor Polyclonal Antibody (SPC-713) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 130kDa. Rabbit Anti-Calcium Sensing Receptor Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-713) | Rabbit Anti-Calcium Sensing Receptor Antibody used in Western blot (WB) on Kidney cell lysates (SPC-713)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
