Immunogen
Synthetic peptide of Human Cyclooxygenase
Antibody's target
Human Cyclooxygenase 1
Antibody's full description
Rabbit Anti-Human Cyclooxygenase 1 Polyclonal
100 µg
SPC-707D-ALP
391€
Synthetic peptide of Human Cyclooxygenase
Human Cyclooxygenase 1
Rabbit Anti-Human Cyclooxygenase 1 Polyclonal
Cardiovascular System, Blood, Platelets, Immunology, Innate Immunity, Macrophage, Cardiovascular System, Atherosclerosis, Cell Signaling, Metabolism, Lipid and lipoprotein Metabolism, Cell Signaling, Metabolism, Redox Metabolism, Metabolism and Oxidative Stress, Cancer, Cancer Metabolism, Cancer Lipid and Lipoprotein Metabolism
Polyclonal Antibodies
EC:1.14.99.1 Antibody, PGHS1 Antibody, Prostaglandin G/H synthase 1 Antibody, PGHS-1 Antibody, Prostaglandin H2 synthase 1 Antibody, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) Antibody, PHS 1 Antibody, EC 1.14.99.1 Antibody, Prostaglandin-endoperoxide synthase 1 Antibody, COX 3 Antibody, PTGS1 Antibody, PGH1_HUMAN Antibody, COX-1 Antibody, PGG/HS Antibody, PTGHS Antibody, Cox3 Antibody, PGH synthase 1 Antibody, Cyclooxygenase-1 Antibody, PHS1 Antibody, Cyclooxygenase 1 Antibody, Cyclooxygenase 3, included Antibody, Partial COX1 proteins, included Antibody, COX 1 Antibody, COX1 Antibody, PCOX1 Antibody
WB
Rabbit
Human, Mouse, Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~75 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-707 was sufficient for detection of Cyclooxygenase in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Microsome Membrane, Peripheral Membrane Protein, Endoplasmic Reticulum Membrane, Peripheral Membrane Protein
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_000953.2
5742
P23219
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa. | Western blot analysis of Mouse Kidney cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa. | Western blot analysis of Rat Brain cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (SPC-707) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa. Rabbit Anti-Cyclooxygenase 1 Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-707) | Rabbit Anti-Cyclooxygenase 1 Antibody used in Western blot (WB) on Kidney cell lysates (SPC-707) | Rabbit Anti-Cyclooxygenase 1 Antibody used in Western blot (WB) on Brain cell lysates (SPC-707)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
