Immunogen
Synthetic peptide from the C-terminal of Human Adenosine receptor A1
Antibody's target
Human Adenosine receptor A1
Antibody's full description
Rabbit Anti-Human Adenosine receptor A1 Polyclonal
100 µg
SPC-702D-ALP
391€
Synthetic peptide from the C-terminal of Human Adenosine receptor A1
Human Adenosine receptor A1
Rabbit Anti-Human Adenosine receptor A1 Polyclonal
Immunology, Innate Immunity, Macrophage, Cardiovascular System, Atherosclerosis, Vascular Inflammation, Inflammatory Mediators, Cardiovascular System, Heart, Cancer, Apoptosis
Polyclonal Antibodies
ADORA1 Antibody, Adenosine receptor A1 Antibody, Adenosine A1 Receptor Antibody, A1AR Antibody, A1R Antibody, AA1R Antibody, ADORA 1 antibody, RDC 7 Antibody, RDC7 Antibody, Ri Antibody, Adenosine receptor (A1) Antibody
WB, ICC/IF
Rabbit
Human
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~37 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-702 was sufficient for detection of Adenosine receptor A1 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cell Membrane, Multi-Pass Membrane Protein
See included datasheet or contact our support service.
Refer to PubMed
25/Oct/2016
NP_000665.1
134
P30542
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Multi-Pass Membrane Protein. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Adenosine receptor A1 Antibody (D) Composite. | Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of ~36.5 kDa Adenosine receptor A1 protein using Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Adenosine receptor A1 Polyclonal Antibody (SPC-702) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~36.5 kDa. Rabbit Anti-Adenosine receptor A1 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-702) | Rabbit Anti-Adenosine receptor A1 Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-702)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
