Immunogen
Synthetic peptide from the mid-protein of Human Ghrelin
Antibody's target
Human Ghrelin
Antibody's full description
Rabbit Anti-Human Ghrelin Polyclonal
100 µg
SPC-696D-ALP
391€
Synthetic peptide from the mid-protein of Human Ghrelin
Human Ghrelin
Rabbit Anti-Human Ghrelin Polyclonal
Cardiovascular System, Blood, Cardiovascular System, Atherosclerosis, Diabetes Associated, Neuroscience, Cardiovascular System, Angiogenesis, Growth Factors
Polyclonal Antibodies
Appetite-regulating hormone Antibody, ghrl Antibody, Ghrelin 28 Antibody, Protein M46 Antibody, GHRL_HUMAN Antibody, GHRL Antibody, Ghrelin Antibody, Growth hormone-releasing peptide Antibody, Motilin-related peptide Antibody, Appetite regulating hormone Antibody, Growth hormone releasing peptide Antibody, Growth hormone secretagogue Antibody, Motilin related peptide Antibody, Ghrelin/obestatin prepropeptide Antibody, Obestatin Antibody, MTLRP Antibody, Ghrelin 27 Antibody, M46 protein Antibody
WB
Rabbit
Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~13 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-696 was sufficient for detection of Ghrelin in 15 µg of rat Pancreas cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Secreted
Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
See included datasheet or contact our support service.
Refer to PubMed
25/Oct/2016
NP_001128413.1
51738
Q9UBU3
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Rat Pancreas showing detection of ~12.9 kDa Ghrelin protein using Rabbit Anti-Ghrelin Polyclonal Antibody (SPC-696). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Ghrelin Polyclonal Antibody (SPC-696) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~12.9 kDa. Rabbit Anti-Ghrelin Antibody used in Western blot (WB) on Pancreas (SPC-696)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Ghrelin, GHRL gene, obestatin prepropeptide or lenomorelin from ghrelinergic cells in the gastrointestinal tract. GHRL is a neuropeptide in the central nervous system regulating appetite. Ghrelin is regulating the distribution and rate of use of body energy.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
