Immunogen
Synthetic peptide of Human PCNA
Antibody's target
Human PCNA
Antibody's full description
Rabbit Anti-Human PCNA Polyclonal
100 µg
SPC-691D-ALP
391€
Synthetic peptide of Human PCNA
Human PCNA
Rabbit Anti-Human PCNA Polyclonal
Cancer, Tumor Biomarkers, Tumor Antigens, Cell Signaling, Epigenetics and Nuclear Signalling, DNA/RNA, DNA Synthesis
Polyclonal Antibodies
cb16 Antibody, MGC8367 Antibody, PCNA_HUMAN Antibody, Polymerase delta accessory protein Antibody, DNA polymerase delta auxiliary protein Antibody, Proliferating cell nuclear antigen Antibody, Mutagen-sensitive 209 protein Antibody, OTTHUMP00000030189 Antibody, PCNAR Antibody, Pcna/cyclin Antibody, HGCN8729 Antibody, wu:fb36g03 Antibody, PCNA Antibody, ATLD2 Antibody, fb36g03 Antibody, OTTHUMP00000030190 Antibody, Cyclin Antibody
WB
Rabbit
Human
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~37 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-691 was sufficient for detection of PCNA in 15 µg of Human 293T Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Nucleus
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_002583.1
5111
P12004
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human 293 Trap cell lysates showing detection of ~28.7 kDa PCNA protein using Rabbit Anti-PCNA Polyclonal Antibody (SPC-691). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PCNA Polyclonal Antibody (SPC-691) at 1:1000 for 1 hour. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 1 hour RT with shaking. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~28.7 kDa. Rabbit Anti-PCNA Antibody used in Western blot (WB) on 293 Trap cell lysates (SPC-691)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus