Immunogen
Synthetic peptide from the mid-protein of Human GAPDH
Antibody's target
Human GAPDH
Antibody's full description
Rabbit Anti-Human GAPDH Polyclonal
100 µg
SPC-689D-ALP
391€
Synthetic peptide from the mid-protein of Human GAPDH
Human GAPDH
Rabbit Anti-Human GAPDH Polyclonal
Neuroscience, Neurodegeneration, Alzheimer’s Disease, Cell Signaling, Metabolism, Cell Signaling, Metabolism, Carbohydrate Metabolism, Cell Signaling, Metabolism, Energy Transfer Pathways, Cancer, Autophagy
Polyclonal Antibodies
CDABP0047 Antibody, GAPD Antibody, GAPDH Antibody, OK/SW-cl.12 Antibody, Peptidyl-cysteine S-nitrosylase GAPDH Antibody, Glyceraldehyde-3-phosphate dehydrogenase Antibody
WB
Rabbit
Human
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~36 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-689 was sufficient for detection of GAPDH in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cytosol, Nucleus, Cytoplasm, Perinuclear Region, Membrane, Cytoplasm, Cytoskeleton
See included datasheet or contact our support service.
Refer to PubMed
25/Oct/2016
NP_001243728.1
2597
P04406
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa cell lysates showing detection of ~36 kDa GAPDH protein using Rabbit Anti-GAPDH Polyclonal Antibody (SPC-689). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GAPDH Polyclonal Antibody (SPC-689) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~36 kDa. Rabbit Anti-GAPDH Antibody used in Western blot (WB) on HeLa cell lysates (SPC-689)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus