Immunogen
Synthetic peptide of Human COX-4
Antibody's target
Human COX-4
Antibody's full description
Rabbit Anti-Human COX-4 Polyclonal
100 µg
SPC-688D-ALP
391€
Synthetic peptide of Human COX-4
Human COX-4
Rabbit Anti-Human COX-4 Polyclonal
Tags and Cell Markers, Organelle Markers or subcellular markers, Cell Signaling, Metabolism, Mitochondrial Metabolism, Mitochondrial Markers, Cancer, Cancer Metabolism, Integration of Energy
Polyclonal Antibodies
COX4I2 Antibody, COX IV Antibody, COX41_HUMAN Antibody, COX4B Antibody, Cytochrome C Oxidase subunit IV Antibody, Cytochrome c oxydase subunit 4 Antibody, COX4I1 Antibody, AL024441 Antibody, COX4 Antibody, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial Antibody, COX IV-1 Antibody, dJ857M17.2 Antibody, Cytochrome c oxidase subunit IV isoform 1 Antibody, COX IV 1 Antibody, COX4L2 Antibody, Cytochrome c oxidase polypeptide IV Antibody, MGC72016 Antibody, COX 4 Antibody, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Antibody, Cytochrome c oxidase subunit 4 isoform 1, COXIV Antibody, Cox4a Antibody, mitochondrial Antibody, Cytochrome c oxidase subunit IV isoform 2 (lung) Antibody, MGC105470 Antibody, Cox4 Antibody
WB, ICC/IF
Rabbit
Human, Mouse, Rat
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~16 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-688 was sufficient for detection of COX-4 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Mitochondrion Inner Membrane
Ubiquitous.
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_001305715.1
1327
P13073
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688). Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Mitochondrion. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) COX-4 Antibody (D) Composite. | Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688). Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Mitochondrion Inner Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) COX-4 Antibody (D) Composite. | Western blot analysis of Human A549 cell lysates showing detection of 19.5 kDa COX-4 protein using Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-COX-4 Polyclonal Antibody (SPC-688) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 19.5 kDa. Rabbit Anti-COX-4 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human SK-N-BE Cells (Human Neuroblastoma cells) (SPC-688) | Rabbit Anti-COX-4 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on NIH 3T3 (Mouse Fibroblast cell line) (SPC-688) | Rabbit Anti-COX-4 Antibody used in Western blot (WB) on A549 cell lysates (SPC-688)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
