Immunogen
Synthetic peptide from the C-terminal of human BNIP3
Antibody's target
Human BNIP3
Antibody's full description
Rabbit Anti-Human BNIP3 Polyclonal
100 µg
SPC-685D-ALP
391€
Synthetic peptide from the C-terminal of human BNIP3
Human BNIP3
Rabbit Anti-Human BNIP3 Polyclonal
Cardiovascular System, Heart, Cancer, Apoptosis, Cell Signaling, Metabolism, Metabolism processes, Hypoxia, Cell Signaling, Metabolism, Mitochondrial Metabolism, Mitochondrial Markers
Polyclonal Antibodies
NIP3 Antibody, BCL2 Adenovirus E1B 19kDa Interacting Protein 3 Antibody, BNIP3_Human Antibody
WB, ICC/IF
Rabbit
Human, Mouse
WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Predicted molecular weight at ~21.5kDa. Observed bands at ~20-30kDa and 50-60kDa due to the homodimer complex of BNIP3.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-685 was sufficient for detection of BNIP3 on mouse kidney lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Mitochondrion, Mitochondrion outer membrane
See included datasheet or contact our support service.
BNIP3 is an apoptosis-inducing protein which can overcome BCL2 suppression. It may play a role in repartioning calcium between the two major intracellular calcium stores in association with BCL2. It has been shown to interact with CD47, BCL2-like, and BCL-2 (1-3).
1. Boyd J.M., et al. (1994) Cell. 79(2): 341-351. 2. Lamy L., et al. (2003) J Biol Chem. 278(26): 23915-23921. 3. Ray R., et al. (2000) J Biol Chem. 275(2): 1439-1448.
1/Oct/2015
NP_004043.3
664
Q12983
Refer to PubMed
To be tested
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Tissue: Fibroblast cell line (NIH 3T3). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BNIP3 Antibody (D) Composite. | Western blot analysis of Human HeLa and 293T cell lysates showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa . | Western blot analysis of Mouse Kidney showing detection of ~30kDa BNIP3 protein using Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685). Lane 1: MW Ladder. Lane 2: Mouse Kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BNIP3 Polyclonal Antibody (SPC-685) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~30kDa . Other Band(s): ~60kDa (BNIP3 complex). Rabbit Anti-BNIP3 Antibody used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Fibroblast cell line (NIH 3T3) (SPC-685) | Rabbit Anti-BNIP3 Antibody used in Western blot (WB) on HeLa and 293T cell lysates (SPC-685) | Rabbit Anti-BNIP3 Antibody used in Western blot (WB) on Kidney (SPC-685)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
