Immunogen
Synthetic peptide from the C-terminal of Human RAB1B (aa. 179-189)
Antibody's target
Human RAB1B
Antibody's full description
Rabbit Anti-Human RAB1B Polyclonal
100 µg
SPC-684D-ALP
391€
Synthetic peptide from the C-terminal of Human RAB1B (aa. 179-189)
Human RAB1B
Rabbit Anti-Human RAB1B Polyclonal
Cell Signaling, Protein Trafficking, Vesicle Transport
Polyclonal Antibodies
Ras-related protein Rab-1B Antibody, RAB1B Antibody
WB
Rabbit
Human, Rat
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~48 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-684 was sufficient for detection of RAB1B in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Membrane, Lipid-Anchor, Cytoplasmic Side, Preautophagosomal Structure Membrane, Lipid-Anchor, Cytoplasmic Side
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_112243.1
81876
Q9H0U4
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 22.1 kDa RAB1B protein using Rabbit Anti-RAB1B Polyclonal Antibody (SPC-684). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAB1B Polyclonal Antibody (SPC-684) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 22.1 kDa. Rabbit Anti-RAB1B Antibody used in Western blot (WB) on HeLa and 293Trap cell lysates (SPC-684)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus