Immunogen
Synthetic peptide from the mid-protein of Human MFN2
Antibody's target
Human Mitofusin 2
Antibody's full description
Rabbit Anti-Human Mitofusin 2 Polyclonal
100 µg
SPC-683D-ALP
391€
Synthetic peptide from the mid-protein of Human MFN2
Human Mitofusin 2
Rabbit Anti-Human Mitofusin 2 Polyclonal
Cell Signaling, Neuroscience, Neurodegeneration, Cell Structure, Cancer, Apoptosis
Polyclonal Antibodies
Transmembrane GTPase MFN2 Antibody, CPRP1 Antibody, MFN2 Antibody, Mitofusin-2 Antibody, EC:3.6.5.- Antibody, KIAA0214 Antibody
WB
Rabbit
Human
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects 86.4 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-683 was sufficient for detection of Mitofusin 2 in 15 µg of human 293Trap cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Mitochondrion Outer Membrane , Multi-Pass Membrane Protein
Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
See included datasheet or contact our support service.
Refer to PubMed
3/Jan/2017
NP_001121132.1
9927
O95140
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human 293Trap cell lysates showing detection of ~86.4 kDa Mitofusin 2 Antibody protein using Rabbit Anti-Mitofusin 2 Antibody Polyclonal Antibody (SPC-683). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Mitofusin 2 Antibody Polyclonal Antibody (SPC-683) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~86.4 kDa. Rabbit Anti-Mitofusin 2 Antibody used in Western blot (WB) on 293Trap cell lysates (SPC-683)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
