Immunogen
Synthetic peptide from the mid to C-terminal of Human IFT88 (aa. 674-686)
Antibody's target
Human IFT88
Antibody's full description
Rabbit Anti-Human IFT88 Polyclonal
100 µg
SPC-679D-ALP
391€
Synthetic peptide from the mid to C-terminal of Human IFT88 (aa. 674-686)
Human IFT88
Rabbit Anti-Human IFT88 Polyclonal
Cell Signaling, Epigenetics and Nuclear Signalling, DNA/RNA, Translation
Polyclonal Antibodies
D13S1056E Antibody, TPR repeat protein 10 Antibody, Tetratricopeptide repeat domain 10 Antibody, Ift88 Antibody, Recessive polycystic kidney disease protein Tg737 homolog Antibody, IFT88_HUMAN Antibody, Polaris homolog Antibody, Probe hTg737 (polycystic kidney disease, autosomal recessive) Antibody, TG737 Antibody, TTC10 Antibody, DAF19 Antibody, IFT88 Antibody, hTg737 Antibody, Intraflagellar transport 88 homolog Antibody, MGC26259 Antibody, Tetratricopeptide repeat protein 10 Antibody, Intraflagellar transport protein 88 homolog Antibody, RP11-172H24.2 Antibody
WB
Rabbit
Human, Mouse
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~94 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-679 was sufficient for detection of IFT88 in 15 µg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cytoskeleton, Microtubule Organizing Center, Centrosome, Centriole, Cytoplasm, Cytoskeleton, Cilium Basal Body
Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
See included datasheet or contact our support service.
Refer to PubMed
29/Aug/2016
NP_001305420.1
8100
Q13099
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human A549 cell lysates showing detection of 94.3 kDa IFT88 protein using Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human A549 cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-IFT88 Polyclonal Antibody (SPC-679) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 94.3 kDa. Other Band(s): 45 kDa. Rabbit Anti-IFT88 Antibody used in Western blot (WB) on A549 cell lysates (SPC-679)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
