Immunogen
Synthetic peptide from the N-terminal to middle of Human RIP3
Antibody's target
Human RIP3
Antibody's full description
Rabbit Anti-Human RIP3 Polyclonal
100 µg
SPC-672D-ALP
391€
Synthetic peptide from the N-terminal to middle of Human RIP3
Human RIP3
Rabbit Anti-Human RIP3 Polyclonal
Cancer, Apoptosis, Cell Signaling, Protein Phosphorylation, Serine / Threonine Kinases, Immunology, Innate Immunity, TLR Signalling
Polyclonal Antibodies
RIPK3 Antibody, Receptor-interacting protein 3 Antibody, Receptor interacting protein 3 Antibody, Receptor interacting serine/threonine protein kinase 3 Antibody, RIP-3 Antibody, RIPK3_HUMAN Antibody, Receptor-interacting serine/threonine-protein kinase 3 Antibody, RIPK 3 Antibody, RIP 3 Antibody, Receptor interacting serine threonine kinase 3 Antibody, RIP3 Antibody, RIP like protein kinase 3 Antibody, RIP-like protein kinase 3 Antibody
WB
Rabbit
Mouse
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~57 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-672 was sufficient for detection of RIP3 in 15 µg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm, Cytosol, Cell Membrane, Mitochondrion
Highly expressed in the pancreas. Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
See included datasheet or contact our support service.
Refer to PubMed
25/Oct/2016
NP_006862.2
11035
Q9Y572
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Mouse Brain cell lysates showing detection of ~56.9 kDa RIP3 protein using Rabbit Anti-RIP3 Polyclonal Antibody (SPC-672). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RIP3 Polyclonal Antibody (SPC-672) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~56.9 kDa. Rabbit Anti-RIP3 Antibody used in Western blot (WB) on Brain cell lysates (SPC-672)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
