Immunogen
Synthetic peptide from the mid-protein of Human ATG3
Antibody's target
Human ATG3
Antibody's full description
Rabbit Anti-Human ATG3 Polyclonal
100 µg
SPC-671D-ALP
391€
Synthetic peptide from the mid-protein of Human ATG3
Human ATG3
Rabbit Anti-Human ATG3 Polyclonal
Neuroscience, Neurodegeneration, Cancer, Tumor suppressors, Cardiovascular System, Heart, Cancer, Autophagy, Cell Signaling, Metabolism, Metabolism processes
Polyclonal Antibodies
ATG3 Antibody, ATG3 autophagy related 3 homolog (S. cerevisiae) Antibody, Ubiquitin-like-conjugating enzyme ATG3 Antibody, APG3-like Antibody, APG3L Antibody, APG3, S. cerevisiae, homolog of Antibody, APG3 like Antibody, Apg 3 Antibody, Autophagy-related protein 3 Antibody, Apg3p Antibody, APG3 Antibody, ATG 3 Antibody, hApg3 Antibody, Autophagy related protein 3 Antibody, Autophagy 3, S. cerevisiae, homolog of Antibody, autophagy related 3 Antibody, ATG3_HUMAN Antibody, Protein PC3-96 Antibody, Autophagy Apg3p/Aut1p like Antibody, PC3 96 Antibody, APG3 autophagy 3 like Antibody, ATG3 autophagy related 3 homolog Antibody
WB
Rabbit
Human
WB (1:1000); optimal dilutions for assays should be determined by the user.
Peptide Affinity Purified
PBS, 50% glycerol, 0.09% sodium azide
1 mg/ml
Detects ~35 kDa.
-20°C
Blue Ice or 4°C
A 1:1000 dilution of SPC-671 was sufficient for detection of ATG3 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cytoplasm
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
See included datasheet or contact our support service.
Refer to PubMed
25/Oct/2016
NP_001265641.1
64422
Q9NT62
Refer to PubMed
To be tested
To be tested
Contact our support service to receive datasheet or other technical documentation.
Western blot analysis of Human HeLa cell lysates showing detection of ~35.9 kDa ATG3 protein using Rabbit Anti-ATG3 Polyclonal Antibody (SPC-671). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-ATG3 Polyclonal Antibody (SPC-671) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~35.9 kDa. Rabbit Anti-ATG3 Antibody used in Western blot (WB) on HeLa cell lysates (SPC-671)
Non-hazardous
Canada
1.4
0.1
In Stock
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
StressMark antibodies supplies antibodies that are for research of human proteins.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Oryctolagus cuniculus
